When Queens popped on the screen the first time I watched, it was like the whole audience changed. They were giggling/reacting to the whole apartment scene.
Ant Man was a riot with the audience pretty much every time he opened his mouth.
Vision got the audience to laugh twice, with both audiences.
Falcon and WS got the audience three times. Twice in the car and once in the airport.
The thing that got the biggest reaction (not laughter) was the slow mo of IM shooting at Cap in the bunker but Cap blocks it with his shield. Ppl started applauding